Figure 5From: Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)Validation of reference genes and tissue-specific analysis of β-pinene synthase expression between CRD, CZ and whole cross-section tissues. (A) Relative mRNA abundance of candidate reference genes normalized to RNA concentration. TIF: Translation initiation factor; ELF: Elongation factor; TUB: Tubulin α-subunit. (B) Relative transcript abundance of β-pinene synthase in different tissue types relative to TIF expression. Error bars represent the standard error of three biological replicates.Back to article page