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Table 1 HPLC-MS2 based identifications of the main anthocyanins detected in petal tissue.

From: Isolation and antisense suppression of flavonoid 3', 5'-hydroxylasemodifies flower pigments and colour in cyclamen

Peak number

Anthocyanin*

Tr (min)

λmax(nm)

[M]+ (m/z)

MS/MS

1

Petunidin 3-O-glucoside;

9.3

277, 343, 526

479

317

2

Malvidin 3-O-glucoside;

14.5

277, 348, 531

493

331

3

Malvidin rhamnosyl-glucoside

15.7

277, 348, 531

639

331, 493

4

Peonidin 3-O-glucoside

13.0

282, 330, 516

463

301

5

Peonidin rhamnosyl-glucoside

14.2

282, 330, 521

609

301,463

6

Malvidin 3,5-di-O-glucoside

8.7

277, 343, 531

655

331, 493

7

Cyanidin 3,5-di-O-glucoside

5.2

282, 516

611

287,449

8

Peonidin 3,5-di-O-glucoside

7.7

277, 330, 516

625

301, 463

  1. * Anthocyanins were identified using HPLC retention times and UV and mass spectrometer data as compared with previously published data.