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Figure 1 | BMC Plant Biology

Figure 1

From: Does the core circadian clock in the moss Physcomitrella patens (Bryophyta) comprise a single loop?

Figure 1

Characterization of putative CCA1/LHY orthologs in P. patens. Characterization of putative CCA1/LHY orthologs in P. patens. (A) Domain structure of CCA1 and LHY in Arabidopsis with the single Myb-domain. (B) An unrooted maximum-likelihood phylogenetic tree constructed from an alignment of the Myb-domain of the SHAQKYF subtype in protein sequences found in A. thaliana, O. sativa, S. moellendorffii, C. reinhardtii and P. patens. (C) Comparative analysis of the oscillating profiles of AtCCA1, PpCCA1a and PpCCA1b in A. thaliana and P. patens. P. patens cultures were sampled every fourth hour for one day in LD (16 h light/8 h dark) and for two days in DD (constant dark). Comparative data for AtCCA1 was downloaded from the DIURNAL database (see Methods). Quantitative RT-PCR expression data was normalized (CTtarget - CTreference) and the minimum transcription level in each time series was arbitrarily set to 0. Grey and white bars surrounded by dashed lines indicate subjective night and day in DD. Plots including error bars indicating standard deviation from duplicate runs of all genes can be found in Additional file 6.

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