Figure 10

Monofunctional wheat SDH ESTs. (A) Wheat ESTs are aligned to the wheat consensus full-length LKR/SDH coding sequence. Intron 14 sequences are shown in blue and green. The sequences of the first seven ESTs have intron 14 spliced out (dashes indicate absent intron sequences). The rest of the ESTs begin with sequence from the middle of intron 14 (bases in blue) to form an exon that is spliced directly to exon 15 in those ESTs. Bases in green are the presumptive transcription initiation signal region. Base differences in red are assumed to indicate homoeologue LKR/SDH sequences of hexaploid wheat. (B) Diagram of exon/intron organization of LKR/SDH bifunctional and SDH monofunctional transcripts. Exons are shown by boxes. The SDH first exon sequence is within the LKR/SDH intron 14 and is shown by the blue box. The green box is the non-transcribed sequence indicated in frame A. Exon numbers are given above (LKR/SDH) and below (SDH) exon boxes.