Figure 7

Wheat LKR/SDH EST alignment. The full-length LKR/SDH coding region derived from BAC 006M07 was used to align wheat ESTs matching with a BLASTn of e^-7 or lower. Red arrows indicate 5' reading ESTs initiating in the LKR or interdomain regions. For the remainder of the ESTs, black arrows show forward 5' reads and blue arrows show reverse 3' reads. Red vertical lines indicate the initial start codon of the LKR domain, the proposed start codon for a monofunctional SDH mRNA, and the common stop codon for both the bifunctional and SDH monofunctional mRNAs. Above the alignment are the numbered exons. Blue boxes indicate the LKR domain and red boxes the SDH domain. Yellow boxes are exons within the interdomain region. The black box represents the short sequence from intron 14 that is found at the 5' end of presumptive monofunctional SDH transcripts and not found in the red-arrowed ESTs. Three 5' and 3' EST pairs from the same clones are labeled as follows: 1, CJ882974 + CJ894783; 2, CJ881957 + CJ893808; 3, CJ883733 + CJ895693.