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Table 1 Proteins identified by MALDI-MS in 2-D IEF-SDS PAGE gels

From: Changes in the proteomic and metabolic profiles of Beta vulgaris root tips in response to iron deficiency and resupply

Spot

Th. MW

Th. pI

Exp. MW

Exp. pI

Score(1,2)

Accession #

Homology

Species

Increased proteins in Fe-deficiency

a

39

7.6

42

5.9

761

T48396

fructose 1,6-bisphosphate aldolase

A. Thaliana

b

27

5.5

35

5.4

1431

gi|556171

triose-phosphate isomerase

C. japonica

c

31

4.9

45

5.4

1031

gi|28172909

cytosolic 3-phosphoglycerate kinase

T. Aestivum

d

49

5.6

59

5.5

1881

gi|1087071

enolase

M. crystallinum

e

49

5.6

58

5.4

1231

T12341

enolase

L. sativa

f

36

5.9

40

6.2

1401

CAB61618

malate dehydrogenase

B. vulgaris

g

36

5.9

40

5.8

771

CAB61618

malate dehydrogenase

B. vulgaris

h

22

7.6

40

6.0

1241

gi|48375044

malate dehydrogenase

N. tabacum

i

55

6.0

60

6.0

1851

O78692

F1 ATPase α subunit

B. vulgaris

j

49

5.1

58

5.3

1701

gi|4388533

F1 ATPase β subunit

S. bicolor

k

36

5.2

49

5.5

2991

gi|1052973

fructokinase

B. vulgaris

l

41

6.5

44

6.3

1021

gi|38636526

formate dehydrogenase

Q. robur

m

26

5.5

35

5.4

1131

gi|21689609

At1g79210/YUP8H12R_1

A. thaliana

n

17

5.9

17

5.5

1162

gi|16301

glycine rich protein

A. thaliana

New spots in Fe-deficiency

o

37

7.1

36

6.6

1011

gi|19566

glyceraldehyde 3-phosphate DH

M. quinquepeta

p

23

8.7

16

6.6

652

Q9XH32

DMRL synthase

S. oleracea

Decreased spots in Fe-deficiency

q

16

6.3

15

6.4

1661

gi|3309053

nucleoside diphosphate kinase I

M. crystallinum

r

23

6.4

30

6.8

2171

gi|11496133

oxalate oxidase-like germin 171

B. vulgaris

s

22

6.1

32

5.7

1881

gi|34365651

At4g27270

A. thaliana

Missing spots in Fe-deficiency

t

23

6.4

34

5.7

2171

gi|11496133

oxalate oxidase-like germin 171

B. vulgaris

u

9

6.0

49

5.7

492

gi|2956703

peroxidase

S. oleracea

v

29

5.1

38

5.3

692

gi|5101868

caffeoyl CoA O-methyltransferase

Z. Mays

  1. Scores (equal to -10*Log P, P being the probability that the observed match is a random event) are based on: (1) MS1 Peptide mass fingerprint data, with protein scores >76 being statistically significant (p < 0.05), and (2)MS2 ion sequencing data, with individual ion scores > 40 indicating identity or extensive homology (p < 0.05). In both cases, protein scores are derived from ion scores as a non-probabilistic basis for ranking protein hits. Proteins are separated in different groups depending on whether they increased, decreased, appeared (new) or disappeared (missing) when compared to control plants.