Figure 4

Powdery mildew infection responsive expression of wheat miRNAs by Northern blot. The tRNA and 5 S RNA bands were visualized by ethidium bromide staining of polyacrylamide gels and served as loading controls. Disease susceptible wheat cultivar Jingdong8 (JD8) and its near-isogenic line Jingdong8-Pm30 (JD8-Pm30) having a resistant gene Pm30 were used. "0 h" means the uninoculated leaves and "12 h" means the leaves were inoculated by the Erysiphe graminis f. sp. tritici (Egt) (Isolate E09).