Figure 3
The orfH79 transgene cassette, and its effect in planta on pollen abortion. A The structure of the binary vectors containing orfH79. Pubi: the maize ubiquitin promoter, Rfo-5': a mitochondrial transit signal from the Raphanus sativus restorer-of-fertility (Ppr-B) gene. B The effect of the transgene on pollen morphology. Darkly stained and lightly stained grains represent, respectively, fertile and sterile pollen. a Pollen from a T0 transformant carrying orfH79. b Pollen from ShijinB. c Pollen from a T0 transformant carrying an empty plasmid. d Pollen from a T1 transformant carrying an inserted orfH79 gene. C Western blot analysis of the mitochondria-enriched protein of the callus. SB, mitochondria protein from callus of ShijinB. U6-1T1,U6-2T1 and U3-2T1, mitochondria protein of callus derived from the three T1 progeny transgenic seeds, respectively. YtA, purified mitochondria protein from etiolated shoots of YtA line. Trans, mitochondrial protein of callus from T1 progeny transgenic seeds.