Figure 5
Analysis of intracellular production of reactive oxygen species (ROS) in transgenic microspores. A Anthers were incubated in 10 μM H2DCFDA for 10 min, and an image of ROS production was captured using confocal microscopy. a and c , Confocal images of T1 transformants carrying orfH79. b and d , Confocal images of non-transgenic plants. a and b , Fluorescent field. c and d , Bright field (bar represents 500 μm). B Production of ROS in microspores of transgenic and wild type plants. ROS content in microspores was quantified after compiling the projections from eight confocal sections of 0.9 μm each (bar represent 100 μm). R.U, relative unit. The data are given as mean ± SE (n = 3).