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Table 1 Segregation of the T1 transgenic plant with orfH7 9

From: The mitochondrial gene orfH79 plays a critical role in impairing both male gametophyte development and root growth in CMS-Honglian rice

Plant lines

Number of positive plant

Number of negative plants

χ2value (for 1:1 segregation)

P

U6-2

15

13

0.14

0.70-0.90

U6-3

10

10

0.05

0.90-0.95

U3-2

17

13

0.3

0.50-0.70

  1. The presence of transgene was determined using PCR. Positive plants: T1 progeny with orfH79 insert, showing a semi-male-sterile phenotype (50% abortion pollens); Negative plants: T1 progeny without orfH79 insert, and showing a fertile phenotype.
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BMC Plant Biology

ISSN: 1471-2229

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