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Figure 5 | BMC Plant Biology

Figure 5

From: Coordinated transcriptional regulation of two key genes in the lignin branch pathway - CAD and CCR - is mediated through MYB- binding sites

Figure 5

GUS expression driven by EgCAD2 and EgCCR promoters containing mutated cis -elements. (A, B) Histochemical analyses of GUS activity, driven by the wild-type or the -203 EgCAD2 and -119 EgCCR promoters mutated in the indicated cis-elements, in stem cross-sections of 8-week-old transgenic tobacco plants. (A) Upper panels, general views of the vascular tissues; lower panels, enlargements of the cambial zone, bars represent 200 ÎĽm and 50 ÎĽm, respectively. CZ, cambial zone; EP, external phloem; IP, internal phloem; PX, primary xylem; RP, xylem ray parenchyma. (B) Upper panels, general views of stem cross-sections; lower panels, enlargements of the vascular tissues. Bars represent 200 ÎĽm. (C) Fluorimetric assays of GUS activity in stems. Histograms represent mean values and standard deviations, expressed as % of the respective wild-type promoter-GUS activities. 7-10 independent transformants were examined for each construct. Activities driven by mutated EgCAD2 and EgCCR promoter are statistically different relative to the control (WT -203 EgCAD2 or WT -119 EgCCR), for MYBb, BSb and MYB Student's P = 9 10-7, 9 10-4 and 0.06, respectively.

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