Figure 6From: Delineating the structural, functional and evolutionary relationships of sucrose phosphate synthase gene family II in wheat and related grassesRelative expression levels of SPSII homoeologues in cv 'Prinz'. Relative expression levels of SPSII shown are based on delta Ct calculation. To compare data from different PCR runs or cDNA samples, CT values for all genes were normalized to the CT value of the housekeeping gene (serine/threonine protein phosphatase, TaPP2A) included in each PCR run. The expression level of each gene of interest (GOI) is presented as 2-ΔCT, where ΔCT = CTGOI - CTREF. Mean values were assessed from three biological replicates for seedling materials and caryopses sampled four days after anthesis, and from two replicates for caryopses sampled eight days after anthesis. Error bars indicate standard deviations from the mean.Back to article page