Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

Table 1 Accession collection of B. rapa and B. oleracea accessions for determining erucic acid contents of seeds and their FAE1 polymorphisms at coding region

Cultivar No.	Cultivar name	Erucic acid content of seeds (%)	Origin	Positions of nucleotides and amino acid polymorphisms
*B. rapa*				591^a	735	898	968	1020	1265
				P197=^b	A245=	R300=	T323I	K340=	F422=
1	Kunshan maquedan	48.58	China	G	C	C	C	G	T
2	Sanyue huang	49.76	China	G	C	C	C	G	T
3	Hanzhong aiyoucai	45.70	China	G	C	C	C	G	T
4	Xinxian huangyoucai	52.02	China	G	C	C	C	G	T
5	7801_1	51.26	China	G	C	C	C	G	T
6	Jumbuck	1.93	Australia	A	T	C	T	A	T
7	Hja 96337	0.90	Finland	A	T	A	T	G	C
*B. oleracea*				489^c	542	1079	1422	1458
				Q163=	T181K	F360S	S474=	Y486=
1	ChunFeng	40.75	China	A	A	T	A	C
2	Zhonggan11	45.90	China	A	A	T	G	C
3	Xiaguang Oleracea	43.27	China	G	C	C	G	T
4	Zhengchun Oleracea	47.63	China	A	A	T	A	C
5	Jingfeng2	48.20	China	A	C	T	G	Y
6	Xinfeng	41.40	China	A	A	Y	G	Y
7	Zaofeng	51.52	China	A	A	Y	A	Y
8	Improved
	Niuxin Oleracea	42.07	China	A	A	T	A	C
9	Hanchun3	41.39	China	G	A	T	G	C

^a591 indicates the position 63 bp from the base 'A' in the start codon of B. rapa FAE1. P197=^b indicates amino acid diversity due to this nucleotide polymorphism. ^c489 indicates the position 63 bp from the base 'A' in the start codon of B. oleracea FAE1. Three haplotypes of FAE1 can be inferred among these seven B. rapa accessions. Accessions Nos. 1-5 are B. rapa haplotype 1; accession No. 6 is B. rapa haplotype 2 and accession No. 7 is B. rapa haplotype 3. Seven haplotypes of FAE1 can be inferred among these nine B. oleracea accessions. Accessions Nos. 1, 4 and 8 are B. oleracea haplotype 1; Accessions No. 2, 3, 5, 6, 7 and 9 are B. oleracea haplotypes 2 to 7, respectively.

ISSN: 1471-2229