Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon

BMC Plant Biology

Table 1 Infectivity of different viruses in 10.6.1 PPV-NK-GFP amplicon plants.

	Inoculated plants^a	Plants displaying disease symptoms	Plants displaying amplicon-derived infection	Recovered plants
10.6.1 + wt PPV	8	5^b	0	5
10.6.1 + TVMV	8	8	0	0
10.6.1 + CMV	8	6	6	0
10.6.1 Mock	8	1	1	1
Wt + wt PPV	4	4	-	0
Wt + TVMV	4	4	-	0
Wt + CMV	4	2	-	0

^a 10.6.1 transgenic (10.6.1) or wild type (wt) N. benthamiana plants were manually inoculated with the indicated viruses. ^bSome PPV CP was detected by western analysis in upper non-inoculated leaves of two asymptomatic plants (Fig. 5A).

ISSN: 1471-2229