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Table 1 Segregation of flower color in F1 plants and F2 population derived from a cross between a soybean cultivar Harosoy with purple flowers and B09121, a Glycine soja accession with light purple flowers, and segregation of flower color in F1 plants, and F2 and F3 populations derived from a cross between a soybean near-isogenic line Clark-w1 with white flowers and B09121 in Tsukuba, Japan.

From: A new allele of flower color gene W1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean Glycine soja

Generation

Year

Number of plants

Expected ratio

χ2 value

Probability

(Pvalue)

  

Total

Purple

Light purple

White

   

B09121 (B)

2007

10

-

10

-

-

-

-

Harosoy (H)

2007

10

10

-

-

-

-

-

H × B F1

2007

3

3

-

-

-

-

-

H × B F2

2007

119

91

28

-

3:1

0.14

0.71

Clark-w1 (w1)

2007

10

-

-

10

-

-

-

w1 × B F1

2007

3

-

3

-

-

-

-

w1 × B F2

2007

124

1

98

25

3:1a

1.43

0.23

w1 × B F3 (purple)b

2008

69

51

18

-

3:1

0.04

0.83

  1. aPlants with light purple flowers: plants with white flowers.
  2. bA line derived from an F2 plant with purple flowers.