Figure 2

Radio-HPLC chromatograms of wounded N. attenuata leaves treated with ¹⁴C-18:3-Glu. (a) Leaves of rosette stage N. attenuata plants were wounded with a pattern wheel and 0.1 μCi of [1-¹⁴C]18:3-Glu were immediately applied onto leaf wounds. The damaged tissue was harvested immediately (T0) or 2 min after the treatment. After extraction, the radiolabeled metabolites were separated by RP-radio-HPLC. The numbers (1 to 3) indicate the different fractions collected for further analysis. CPM: counts per minute. (b) M. sexta OS was spiked with 0.1 μCi ¹⁴C 18:3-Glu, applied onto wounded leaf tissue, extracted after different time points and separated by TLC (W+OS). Plates were exposed for 24 h to ¹⁴C-sensitive screens. Time 0: ¹⁴C 18:3-Glu was applied onto wounded leaves and immediately extracted. W+: ¹⁴C-18:3-Glu was applied onto wounded leaves and extracted after 2 min. 18:3-Glu^m1 and 18:3-Glu^m2 indicate different modified forms of 18:3-Glu.