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Figure 5 | BMC Plant Biology

Figure 5

From: The two α-dox genes of Nicotiana attenuata: overlapping but distinct functions in development and stress responses

Figure 5

Silencing Na α-dox1 and/or Na α-dox2 in Nicotiana attenuata differentially affects defense 2-HOT, hormone and nicotine accumulation. Mean ± SE (n = 6 induced & 5 constitutive plants) of leaf levels of (A) 2-HOT, (B) ABA, (C) A, and (D) nicotine of plants that had been fed on by Manduca sexta larvae for 4 days or left undamaged. Plants were transiently silenced for Naα-dox1 and Naα-dox2 by virus-induced gene silencing (VIGS) or transformed with an empty vector (ev). Wild-type plants (WT) and plants stably silenced for Naα-dox1 using an inverted repeat (IR) construct were inoculated with VIGS constructs when 22 days old. Larvae were applied on day 39. Two-factorial ANOVAs with factor transformant and factor M. sexta feeding revealed significant effects of (2-HOT levels: transformant F4,40 = 23.27, M. sexta F1,40 = 203.41, interaction F4,40 = 5.07, all P < 0.002; ABA levels: transformant F4,35 = 10.53, P < 0.001, M. sexta F1,35 = 8.08, P = 0.007, interaction F4,35 = 0.82, P = 0.52; JA levels: transformant F4,35 = 10.15, P < 0.0001, M. sexta F1,35 = 1058.2, P < 0.0001, interaction F4,35 = 0.41, P = 0. 80). Different letters signify significant differences according to 1-factorial ANOVAs and Fisher's PLDS at P < 0.05 (ANOVA P and F values are given in the graph).

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