Figure 4
Transcript levels of JA marker genes in N, P and Ca deficient plants. Northern blot analysis of LOX2 and VSP2 transcript level in response to different nutrient deficiencies. Plants were grown for 14 days on vertical Petri dishes containing a control medium (control, C) or media with low levels of nitrogen (-N), phosphorus (-P), calcium (-Ca) or potassium (-K) (see Additional files 2 and 3 for media composition and plant appearance). LOX2 and VSP2 signals were obtained after hybridization with the corresponding 32P-labelled probes and autoradiograms are presented. rRNA levels after a methylene blue staining show the total amount of RNA in each sample blotted onto the membrane. Note that due to the strong accumulation of LOX2 and VSP2 transcripts in low-K plants less RNA was loaded onto the gel.