Figure 4
From: Modulation of redox homeostasis under suboptimal conditions by Arabidopsis nudix hydrolase 7
Western analysis using polyclonal antibodies raised against recombinant AtNUDT7 protein. A. WT Col-0 plants were infected with 1 × 106 CFU/mL of Pseudomonas syringae avrRpt2. Leaves were harvested immediately after infiltration (0), and at 2, 4, 6, 8, and 24 hours post-infection For each lane, 20 μg of total protein was used. B. Three-week old Wassilewskija ecotype plants were fumigated with 250 nL. L-1 of ozone for six hours. Rosettes were collected at 0.5, 1, 2, 3, and 4 hours during ozone treatment and 24 hours after the initiation of treatment. Control (C) plants were maintained under ambient ozone conditions. For each lane, 20 μg of total protein was used. C. AtNUDT7 protein levels were monitored in WT Col-0 plants eight hours after wounding by a sharp blade. For each lane, 20 μg of total protein was used.