Figure 9
From: Modulation of redox homeostasis under suboptimal conditions by Arabidopsis nudix hydrolase 7
Biochemical assays to support the microarray based gene expression data in the Atnudt7-1 mutant. A. Protease activity assay in WT Col-0 and Atnudt7-1 mutant plants using azocasein as substrate. Leupeptin (0.1 mM) and phenylmethyl sulfonate (PMSF; 1 mM) were added to protein extracts from WT and mutant plants. The assay was repeated four times, twice from each of the two biological replicates of plants. Bars with different letters indicate statistically significant differences in protease activity compared with WT. B. Analysis of ATP content in WT Col-0 and Atnudt7-1 plants. The assay was repeated four times, twice from each of the two biological replicates of plants. Bars with different letters indicate statistically significant differences in ATP levels in Atnudt7-1 mutant compared with WT.