Skip to main content
Figure 1 | BMC Plant Biology

Figure 1

From: Asymmetric localization of Arabidopsis SYP124 syntaxin at the pollen tube apical and sub-apical zones is involved in tip growth

Figure 1

Confocal imaging of N. tabacum pollen tubes expressing GFP-AtSYP124. Scale bars = 20 μm. A: Optical section of a growing pollen tube transformed with free GFP showing a cytosolic distribution. B: Time-course series of a growing tobacco pollen tube transiently transformed with the GFP-SYP124 construct showing the subcellular localization of SYP124 (observation made ~6 h after transformation). The protein seemed to accumulate mostly in apical vesicles and at the flanks of the plasma membrane near the pollen tip. Numbers refer to the time interval (in seconds). Images are representative of twelve independent experiments. C: Optical section of a non-growing pollen tube transformed with GFP-SYP124. D: Pollen tube expressing high levels of GFP-SYP124 exhibited disturbed growth and perturbations in apical morphology. The protein accumulates at the flanks but also at the apical plasma membrane. E: Time course analysis of GFP fluorescence intensity in the growing pollen tube shown in B. Each line is representative of measurements of the fluorescent signal made in the lines/circles depicted in the diagram for 12 different pollen tubes: apical plasma membrane (black circle); apical cytoplasm (black square); sub-apical plasma membrane (lozenge); sub-apical cytoplasm (triangle). Growth rate measurements (μm.min-1) are displayed by the dashed line. F: Distribution of average fluorescence intensity along the first 40 μm of the pollen tubes plasma membrane as depicted in the diagram for growing (Grow - solid line; n = 12), non-growing (Non-grow - dashed line; n = 3) and pollen tubes over-expressing GFP-SYP124 (Over-exp - dotted line; n = 5). Differences are statistically significant (P = 5E-9). Error bars not displayed for the sake of clarity.

Back to article page