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Table 1 Amplification procedure of AmCBL1 promoter

From: Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus

Step

procedure

primers

template

First intron amplification

INT1/INT2

genome DNA

First walking

linear amplification reaction add poly C to end

GSP1

genome DNA

 

amplification of poly-added production

GSP2/AAP

poly-added production

 

amplification of target gene

GSP3/AUAP

last step production

PCR amplification

W1/GSP2

genome DNA

second walking

linear amplification reaction add poly C to end

GSP4

genome DNA

 

amplification of poly-added production

GSP5/AAP

poly-added production

 

amplification of target gene

GSP6/AUAP

last step production

PCR amplification

W1/GSP2

genome DNA

third walking

linear amplification reaction add poly C to end

GSP7

genome DNA

 

amplification of poly-added production

GSP8/AAP

poly-added production

 

amplification of target gene

GSP9/AUAP

last step production

AmCBL1 promoter amplification

S1/GSP2

genome DNA