Step | procedure | primers | template |
---|---|---|---|
First intron amplification | INT1/INT2 | genome DNA | |
First walking | linear amplification reaction add poly C to end | GSP1 | genome DNA |
 | amplification of poly-added production | GSP2/AAP | poly-added production |
 | amplification of target gene | GSP3/AUAP | last step production |
PCR amplification | W1/GSP2 | genome DNA | |
second walking | linear amplification reaction add poly C to end | GSP4 | genome DNA |
 | amplification of poly-added production | GSP5/AAP | poly-added production |
 | amplification of target gene | GSP6/AUAP | last step production |
PCR amplification | W1/GSP2 | genome DNA | |
third walking | linear amplification reaction add poly C to end | GSP7 | genome DNA |
 | amplification of poly-added production | GSP8/AAP | poly-added production |
 | amplification of target gene | GSP9/AUAP | last step production |
AmCBL1 promoter amplification | S1/GSP2 | genome DNA |