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Table 1 Average silent site divergence (K s ) and estimated age of split (bold) between M. truncatula and related species from the vicioide subclade, using three chloroplast gene regions: rbcL, matK and trnL

From: Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds

 

Melilotus

Trifolium

Pisum

Vicia

Medicago truncatula

 

rbcL

  
 

(0.0590)

9.1 My

M. albus

(0.0882)

13.6 My

T. pratense

(0.1194)

18.5 My

P. sativum

(0.1556)

24.1 My

V. cracca

  

matK

  
 

(0.0511)

6.7 My

M. albus

(0.0661)

8.7 My

T. albopurpureum

(0.1149)

15.2 My

P. sativum

(0.1103)

14.6 My

V. hirsuta

  

trnL

  
 

(0.0126)

6.6 My

M. albus

(0.0254)

13.2 My

T. praetermissum

(0.0211)

11.0 My

P. sativum

(0.0297)

15.5 My

V. nipponica

Average Age

of split

7.5 My

11.8 My

14.9 My

18.1 My

  1. As a calibration point we used the 34 million years estimate for the split between Pisum and Albizia species [27] and the levels of synonymous divergence per synonymous site (K s ) for the published chloroplast genes rbcL, matK and trnL of Pisum sativum and three Albizia species, namely the K s values of 0.2199 for P. sativum/A. julibrissin; 0.2575 for P. sativum/A. versicolor and 0.0653 for P. sativum/A. julibrissin, respectively.