Skip to main content
Figure 6 | BMC Plant Biology

Figure 6

From: Translation reinitiation and development are compromised in similar ways by mutations in translation initiation factor eIF3h and the ribosomal protein RPL24

Figure 6

The 5' leaders of many uORF-containing ARF mRNAs render translation dependent on eIF3h. (A) Schematic view of the mRNAs for protoplast transformation. Open arrows represent RLUC ORF, hatched arrows represent codon-optimized FLUC (LUC+). mRNAs were prepared by in vitro transcription with SP6 RNA polymerase. An equal amount of internal control (Spacer-LUC+) mRNA was added to the 5' leader-RLUC mRNA to be tested as an internal control for transformation efficiency. (B and C) Translational efficiency on the given ARF 5' leader is expressed as the mean RLUC/FLUC ratio with standard errors from three replicate transformations. Data from several other auxin related 5' leaders are shown for comparison. PIN1 does not contain uORFs. AUX1 and TIR1 each have one short uORF of six codons.

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us