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Figure 2 | BMC Plant Biology

Figure 2

From: The Arabidopsis pop2-1mutant reveals the involvement of GABA transaminase in salt stress tolerance

Figure 2

GABA metabolism regulation upon NaCl treatment. Ten-day-old plantlets of wild-type (WT, Ler accession) grown on agar medium were transferred to agar medium supplemented, or not (Control), with NaCl. (A-B) Time-course and organ partitioning of GABA content during NaCl treatment. GABA content was determined either in whole plantlets treated with 150 mM NaCl over an 8-day-period (A) or in shoots and roots of plantlets after 4 days of treatment with 150 mM NaCl (B). Results are the mean ± S.E. of 3 independent replicates. (C-F) Time-course and dose-response of GAD and GABA-TP activities upon NaCl. Glutamate decarboxylase activity (GAD, D-E) and GABA transaminase activity using pyruvate as GABA amino group acceptor (GABA-TP, F-G) were determined in entire plantlets either over a 4-day-period of treatment with 150 mM NaCl (D and F) or after 4 days of treatment with increasing concentration of NaCl (E and G). Results are the mean ± S.E. of 4-10 independent replicates. (G) Dose-response of GABA metabolism genes to increasing concentration of NaCl after 24 h of treatment. Total RNA was isolated from whole plantlets and served to gene expression analysis of the five glutamate decarboxylase (GAD1-5), the GABA transaminase (POP2), the succinate semialdehyde dehydrogenase (SSADH) and the well-known stress-induced Δ1-pyrroline-5-carboxylate synthetase 1 (P5CS1). Results are the mean ± S.E. of 3 independent replicates. nd, not detected. Stars indicate a significant difference with control according to non-parametric Mann-Whitney U-test (P < 0.05)

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