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Table 1 Modified nucleosides in Arabidopsis, Populus compared to S. cerevisiae.

From: Transfer RNA modifications and genes for modifying enzymes in Arabidopsis thaliana

Nucleosidesa Arabidopsis & Populus S. cerevisiae Calf liver E. coli
Db +b +b +b +b
Ψ + + + +
C + + + +
cmo5U - - - +
ncm5U + + + +
U + + + +
m3C - + + -
s2C - - - +
m1A + + + -
m5C + + + -
mnm5s2U - - - +
Cm + + + +
m7G + + + +
m5U + + + +
I + + + +
G + + + +
Qg n.a - + +
acp3U - - - +
s4U - - - +
Um + + + +
m1I + + + -
mcm5Uc - + n.a. n.a.
Gm + + + +
m1G + + + +
m2G + + + -
ac4C + + - -
A + + + +
m22G + + + -
mcm5s2U - + n.a. n.a.
Am + + + -
t6A + + + +
m2A + - - +
m6A + + + +
m6t6A + - + +
YOH - + + -
io6A - - + +
ms2io6A + - - -f
Y - + - -
Ar(p)d n.a. + n.a. n.a.
ncm5Ume n.a. + n.a. n.a.
  1. a. Nucleosides are listed in the order of retention time in HPLC chromatogram, threshold for detection is approximately 0.002% of total area in HPLC chromatogram, any modified nucleoside below this threshold is designated as "-".
  2. b. D (dihydrouridine) is not easily detected in HPLC system because of the very early retention time and because of its close elution with Ψ.
  3. c. mcm5U is detected in yeast single tRNA prep but not in bulk tRNA preps.
  4. d. Ar(p) is present on initiator tRNA in S. cerevisiae (Aström SU, 1994), its position in HPLC is unknown.
  5. e. ncm5Um is detected in yeast single tRNA prep (Glasser AL, 1992), its position in HPLC is unknown.
  6. f. E. coli has ms2i6A but Salmonella enterica has ms2io6A.
  7. g. Q is not being analyzed because it is destroyed during tRNA extraction and digestion.
  8. n.a. Data not available.