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Table 1 Success ratios of PCR amplification and sequencing for seven candidate barcoding markers.

From: DNA barcoding of the Lemnaceae, a family of aquatic monocots

 

psbK-psbI

trnH-psbA

matK

atpF-atpH

rpoB

rpoC1

rbcL

Max. length of product*

576

504

725

622

389

450

522

Min. length of product*

185

286

719

579

389

450

522

# tested Samples

97

97

97

97

97

97

97

% Success of PCR and sequencing

100%

95%

71%

99%

98%

100%

100%

  1. * The analyzed product length becomes shorter than corresponding one's due to removal of the end of ambiguous nucleotides