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Table 5 List of primers for the seven proposed DNA barcoding markers.

From: DNA barcoding of the Lemnaceae, a family of aquatic monocots

Marker

Primer sequence

Amplicon size (Lemna minor)

Ta Optimum (Lemna minor)

psbK-psbI

Forward: 5'-TTAGCATTTGTTTGGCAAG-3';

544 bp

51°C

 

Reverse: 5'- AAAGTTTGAGAGTAAGCAT -3'

  

trnH-psbA

Forward: 5'-GTTATGCACGAACGTAATGCTC-3';

300 bp

55°C

 

Reverse: 5'- CGCGCGTGGTGGATTCACAATCC-3'

  

matK

Forward: 5'-CGTACTGTACTTTTATGTTTACGAG-3';

862 bp

55°C

 

Reverse: 5'- ATCCGGTCCATCTAGAAATATTGGTTC -3'

  

atpF-atpH

Forward: 5'-ACTCGCACACACTCCCTTTCC-3';

675 bp

53°C

 

Reverse: 5'- GCTTTTATGGAAGCTTTAACAAT -3'

  

rpoB

Forward: 5'-ATGCAGCGTCAAGCAGTTCC-3';

406 bp

55°C

 

Reverse: 5'- TCGGATGTGAAAAGAAGTATA -3'

  

rpoC1

Forward: 5'-GGAAAAGAGGGAAGATTCCG-3';

509 bp

56°C

 

Reverse: 5'- CAATTAGCATATCTTGAGTTGG -3'

  

rbcL

Forward: 5'-GTAAAATCAAGTCCACCACG-3';

580 bp

56°C

 

Reverse: 5'-ATGTCACCACAAACAGAGACTAAAGC -3'

  
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BMC Plant Biology

ISSN: 1471-2229

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