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Table 1 List of accepted substrates for CYP75A31

From: Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum

Substrate

Product of 3'-hydroxylation

Product of 5'-hydroxylation

Class

Luteolin

(20.3) [286]

-

Tricetin

(18.2) [302]

Flavone

Naringenin

(21.2) [272]

Eriodictyol

(19.1) [288]

5,7,3',4',5'-pentahydroxyflavanone

(16.3)

Flavanone

Eriodictyol

(18.9) [288]

-

5,7,3',4',5'-pentahydroxyflavanone (16.2) [304]

Flavanone

Dihydrokaempferol (17.0) [288]

Dihydroquercetin

(15.0) [304]

Dihydromyricetin

(12.4) [320]

Dihydroflavonol

Dihydroquercetin (15.0) [302]

-

Dihydromyricetin

(12.4) [318]

Dihydroflavonol

Kaempferol

(22.4) [286]

Quercetin

(20.1) [302]

Myricetin

(17.1) [318]

Flavonol

Quercetin

(20.0) [302]

-

Myricetin

(17.0) [318]

Flavonol

Liquiritigenin

(19.0) [256]

Butin

(17.03) [272]

7,3',4',5'-tetrahydroxyflavanone

(14.4)

Flavanone

  1. Enzyme assays were run on microsome preparations of yeast transformed with the CYP75A31 gene. Product formation was analysed by HPLC and MS. HPLC retention times in minutes are given in parenthesis. Masses in g/mol, as determined by MS, are given in brackets.