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Table 1 List of accepted substrates for CYP75A31

From: Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum

Substrate Product of 3'-hydroxylation Product of 5'-hydroxylation Class
Luteolin
(20.3) [286]
- Tricetin
(18.2) [302]
Flavone
Naringenin
(21.2) [272]
Eriodictyol
(19.1) [288]
5,7,3',4',5'-pentahydroxyflavanone
(16.3)
Flavanone
Eriodictyol
(18.9) [288]
- 5,7,3',4',5'-pentahydroxyflavanone (16.2) [304] Flavanone
Dihydrokaempferol (17.0) [288] Dihydroquercetin
(15.0) [304]
Dihydromyricetin
(12.4) [320]
Dihydroflavonol
Dihydroquercetin (15.0) [302] - Dihydromyricetin
(12.4) [318]
Dihydroflavonol
Kaempferol
(22.4) [286]
Quercetin
(20.1) [302]
Myricetin
(17.1) [318]
Flavonol
Quercetin
(20.0) [302]
- Myricetin
(17.0) [318]
Flavonol
Liquiritigenin
(19.0) [256]
Butin
(17.03) [272]
7,3',4',5'-tetrahydroxyflavanone
(14.4)
Flavanone
  1. Enzyme assays were run on microsome preparations of yeast transformed with the CYP75A31 gene. Product formation was analysed by HPLC and MS. HPLC retention times in minutes are given in parenthesis. Masses in g/mol, as determined by MS, are given in brackets.