Table 2 Primers used in this study
Name | Sequence (5' to 3') | Use |
|---|---|---|
1gRTF | GCACCATTGTAGAGCAGGTCA | Semi-quantitative |
1gRTR | ACCAATCGCTTCTTGATCGTC | RT- PCR |
3gRTF | TACATACCGCCAACGAGTGAT | Semi-quantitative |
3gRTR | GCTACAAGAACCGTATCGAAG | RT- PCR |
5gRTF | TCAAAGCAAGTCCGGAGGAGT | Semi-quantitative |
5gRTR | TTTGGTCAGCCCATCATTGTC | RT- PCR |
TuRTF | AGAACACTGTTGTAAGGCTCAAC | Semi-quantitative |
TuRTR | GAGCTTTACTGCCTCGAACATGG | RT- PCR |
5gNcoI F | CGGCCATGGAAATGCATTCGCCTCTTCAGACTCA | pICME-GFP construction |
5gNcoI R | TTGCCATGGCGAAAGGGCTAATCTCACGAG | Â |
1gBglII F | GCCAGATCTATGCCGTCGCAGATTCTCCAAAT | pGFP-ICME-LIKE1 construction |
1gSpeI R | GTGACTAGTTAGAACGGACTGACCCAGTGAGCCAACTT | Â |
3gBglII F | GTCTAGATCTATGCAGTTGTCTCCGGAACG | pGFP-ICME-LIKE2 construction |
3gSpeI R | TTAACTAGTCAAAAAGGGCTGACCCTGCCAGCCAGTT | Â |
5gΔTM F1 | GAAATGCATTCGCCTCTTCAG | pICMEΔTM-GFP construction |
5gΔTM R1 | CGGACTTGCTTTGAGAAAAATTTTGTCATCCATCGATAGC |  |
5gΔTM F2 | GCTATCGATGGATGACAAAATTTTTCTCAAAGCAAGTCCG |  |
5gΔTM R2 | AAGGCATGCTACAATGATATC |  |
1gqRTF | CTCACATCATCTTCCACCTAAATC | Real-time quantitative |
1gqRTR | AAATCGAGAGAGAAGGGTCGT | PCR |
3gqRTF | CCGATGTCTGAAAACAGAGAGG | Real-time quantitative |
3gqRTR | CCGGTGAAGATAATCTGTTGG | PCR |
5gqRTF | AGGATCCCTTACGAGGAGGT | Real-time quantitative |
5gqRTR | AGCCAACGAGTCTTTGGTCAG | PCR |
TuqRTF | GTGCTGAAGGTGGAGACGAT | Real-time quantitative |
TuqRTR | AACACGAAGACCGAACGAAT | PCR |
Actin8qRTF | TTACCCGACGGACAAGTGATC | Real-time quantitative |
Actin8qRTR | ATGATGGCTGGAAAAGGACTTC | PCR |
P2 | tcacttccataatcggggtctg | T-DNA screening |
LBa1 | TggTTCACgTAgTgggCCATCg | T-DNA screening |