Figure 3From: Overexpression of a natural chloroplast-encoded antisense RNA in tobacco destabilizes 5S rRNA and retards plant growthAS5 transcript analysis. (A) Extent of the AS5 transgene (black bar) in relation to rrn5, trnR, and representative tobacco and tomato AS5 EST's (solid black arrows). Locations of the strand-specific RNA probes (P1-P3) used in (B) are shown above (dashed black arrows). ESTs are: 1 - tobacco AM826917, 479 nt; 2 - tomato AJ85186, 785 nt; 3 - tomato BI422136, 774 nt; 4 - tomato EST587142, 787 nt; 5 - tomato AJ832014, 696 nt. (B) 10% polyacrylamide gel blot analysis of AS5 in WT and AS5ox-1 (1) and AS5ox-2 (2). The probes used are shown at the top of the blot, and four WT bands (WT1-4) and an AS5ox-specific band (AS5ox) are indicated with arrows at the left. Two exposures are shown for the P2 probe to confirm bands WT1-3 and illustrate the abundance of WT4. Size standards are shown at the right.Back to article page