Table 4 Information about eleven pairs of primers for PCR amplification and the length of their products
From: The origin of populations of Arabidopsis thalianain China, based on the chloroplast DNA sequences
Primer No. | Intergenic region | Site* (nt) | Primer sequence | GenBank Acc No |
|---|---|---|---|---|
1 | trnR-atpA | 9662-9937 | 5'-GGATAGGACATAGGTCTTCTAA-3' 5'-CACAGTGGAAGAACAGATAATG-3' | GU293237-GU293316 |
2 | rpoB-trnC | 26330-27372 | 5'-CCCTTCAAATTGTATCTGATTAAA-3' 5'-GATTTGAACTGGGGAAAAAGGATT-3' | GU293317-GU293396 |
3 | trnG-trnfM-rps14 | 36561-36937 | 5'-GCGGATATAGTCGAATGGTAAA-3' 5'-GTAAGATTCCGTCGCTAAGTGA-3' | GU293397-GU293476 |
4 | ycf3-trnS | 43752-44826 | 5'-CGCATAGCTTCATAATAATTCTGT-3' 5'-TCTACATAACAGTTCCAATGTTAC-3' | GU293477-GU293556 |
5 | trnL-trnF | 47491-48174 | 5'-TCCTCTGCTCTACCAACTGA-3' 5'-AAATCGTGAGGGTTCAAGTC-3' | GU293557-GU293636 |
6 | rbcL-accD | 56398-57074 | 5'-CTAGCTGCTGCTTGTGAAGTATGG-3' 5'-TAAAATTGAACCACGATTTTTCCA-3' | GU293637-GU293716 |
7 | accD-psaI | 58542-59246 | 5'-CAATTGCCGGAAAGACTAGG-3' 5'-GTTCACAAGCGGCTGAATCT-3' | GU293717-GU293796 |
8 | psbE-ORF31 | 64323-65711 | 5'-TATCGAATACTGGTAATAATATCA-3' 5'-ATAGTTAAAGCTGCTAGTAGAAAA-3' | GU293797-GU293876 |
9 | psaJ-rpl33 | 67064-67487 | 5'-CTAGGGGTGTTATGCCGATT-3' 5'-GTTCGGTTCGTTAGCAGGTT-3' | GU293877-GU293956 |
10 | rpl20-clpP | 68866-69909 | 5'-CATGGAACGGGATGTTTTTA-3' 5'-GTTCTACGCCTCCGAGCTAT-3' | GU293957-GU294036 |
11 | Intron in rpl16 | 81588-82643 | 5'-TCCTCGATGTTGTTTACGAAATCT-3' 5'-TCGAACTATTTATGGGGTTTTAGG-3' | GU294037-GU294116 |