Figure 3

Analysis of selected At GRP7 candidate target transcripts in transgenic lines with elevated or reduced At GRP7 levels. A) AtGRP7-ox and wt plants were grown in LDs and harvested around the circadian maximum. Expression levels were determined by qRT-PCR and normalized to PTB expression. Shown is the expression in AtGRP7-ox plants relative to wt. B) Transcript levels of the JA-responsive PDF1.2a in MeJ-treated plants. Independent AtGRP7-ox lines and the corresponding wt plants were grown in liquid culture in LDs and harvested 24 h after MeJ treatment. Expression levels were determined by qRT-PCR and normalized to PTB. The level in untreated wt plants is set to 1. C) Mutation of a conserved arginine in the AtGRP7 RNA recognition motif interferes with regulation of target transcripts. qRT-PCR was performed on RNA from AtGRP7-RQ-ox and wt plants grown in LDs and harvested at zt12 for the analysis of PR1, PR2, THI2.2 and RD29A, and at zt3 for the analysis of HYH and STH, respectively. Data were normalized to PTB and the levels in AtGRP7-RQ-ox plants are expressed relative to wt. D) qRT-PCR was performed on RNA from the T-DNA line atgrp7-1 and Col-0 wt plants grown in LDs and harvested at zt12 for the analysis of PR1, PR2, THI2.2, PDF1.1, PDF1.2a and RD29A, and at zt3 for the analysis of HYH and STH, respectively. Data were normalized to PTB and the levels in atgrp7-1 are expressed relative to wt.