The physical and functional borders of transit peptide-like sequences in secondary endosymbionts

BMC Plant Biology

Table 1 Constructs investigated in this study

Construct name	Protein sequence^a	Net charge^b	Localization^c
FcpD-eGFP	... FAPA K NAA R TSVATT-MVS K ...	+3	S
FcpD-eGFP_K4E	... FAPA K NAA R TSVATT-MVS E ...	+1	S
FcpD-eGFP_K4A	... FAPA K NAA R TSVATT-MVS A ...	+2	S
FcpD_K20E+R24E-eGFP_K4E	... FAPA E NAA E TSVATT-MVS E ...	-3	ER
FcpD_K20R-eGFP_K4R	... FAPA R NAA R TSVATT-MVS R ...	+3	S
FcpD_K20A+R24A-eGFP_K4A	... FAPA A NAA A TSVATT-MVS A ...	0	PPC
FcpD_K20E+R24E-eGFP	... FAPA E NAA E TSVATT-MVS K ...	-1	ER/PPC
FcpD_K20E+N21K+R24E-eGFP	... FAPA EK AA E TSVATT-MVS K ...	0	PPC
FcpD_K20E+N21K+R24E-eGFP_K4E	... FAPA EK AA E TSVATT-MVS E ...	-2	ER
FcpD_K20A+R24A-eGFP	... FAPA A NAA A TSVATT-MVS K ...	+1	PPC
FcpD_K20E+N21R+R24E-eGFP_K4R	... FAPA ER AA E TSVATT-MVS R ...	0	PPC
FcpD_K20E+N21R+R24E-eGFP_K4E	... FAPA ER AA E TSVATT-MVS E ...	-2	ER
FcpD_Δ20-30-eGFP	... FAPA-MVS K ...	+1	S
FcpD_Δ20-30-eGFP_K4R	... FAPA-MVS R ...	+1	S
FcpD_Δ20-30-eGFP_K4E	... FAPA-MVS E ...	-1	ER
FcpD_Δ20-30-eGFP_K4A	... FAPA-MVS A ...	0	PPC
FcpD_3xSpacer-eGFP	... FAPA K NAA R TSVATT-[M(GGGGP)₅]₃-MVS K ...	+2	S
FcpD_Δ20-30-Spacer-eGFP	... FAPA-[M(GGGGP)₅]-MVS K ...	0	S
FcpD_Δ20-30-2xSpacer-eGFP	... FAPA-[m(GGGGP)₅]₂-MVS K ...	0	S
FcpD_Δ20-30-3xSpacer-eGFP	... FAPA-[M(GGGGP)₅]₃-MVS K ...	0	ER
FcpD_Δ20-30-3xSpacer_G4K-saGFP11	... FAPA-[M(GG K GP)-(GGGGP)₄]₃-MVS K ...	+1	ER/S

^aSequence of the FcpD transit peptide, of the eGFP N-terminus and, in spacer constructs, of the synthetic spacers separated by hyphens, respectively; charged amino acids (or substituted alanines) in bold; ^bCalculated from the bold-faced amino acids, in spacer constructs up to the end of the first spacer; ^cS: stroma; ER: endoplasmic reticulum; PPC: periplastid compartment.

ISSN: 1471-2229