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Table 2 Experimental Functional Annotation of VvTPS Genes of the VvTPS-b Subfamily

From: Functional Annotation, Genome Organization and Phylogeny of the Grapevine (Vitis vinifera) Terpene Synthase Gene Family Based on Genome Assembly, FLcDNA Cloning, and Enzyme Assays

Functional Gene ID

Function

Access. #

FLcDNA ID

Nearest VvTPS gene model

Representative Products

%

VvGwaPhe

(+)-α-Phellandrene Syn

HM807382

Gw74ME

VvTPS45

(+)-α-phellandrene

40

     

myrcene

15

     

terpinolene

15

     

α-terpinene

7

     

γ-terpinene

6

     

(+)-limonene

5

     

(+)-α-pinene

3

     

(+)-β-phellandrene

3

     

(+)-α-terpineol

3

     

(E)-β-ocimene

2

VvPNaPin1

(+)-α-Pinene Syn

HM807383

PN20M1

VvTPS44

(+)-α-pinene

47

VvPNaPin2

 

HM807384

PN05S14

VvTPS44

(+)-limonene

25

     

(+)-camphene

9

     

myrcene

6

     

(+)-α-terpineol

5

     

(+)-sabinene

3

     

(3S)-linalool

2

     

(+)-β-pinene

1

     

(+)-α-phellandrene

1

     

(+)-α-thujene

1

     

α-terpinolene

1

VvGwbOci

(E)-β-Ocimene syn

HM807385

Gw22YB2

VvTPS34

(E)-β-ocimene

98

VvCSbOci

 

HM807386

CS402F

VvTPS35

(Z)-β-ocimene

2

VvCSbOciM

(E)-β-Ocimene/Myrcene syn

HM807387

CS19M

VvTPS38

(E)-β-ocimene

53

     

myrcene

42

     

β-pinene

3

     

limonene

2

VvGwbOciF

(E)-β-Ocimene/(EE)-α-Farnesene Syn

HM807388

Gw46YB3

VvTPS47

(E)-β-ocimene

100*

VvCSbOciF

 

HM807389

CS93F

VvTPS47

(E,E)-α-farnesene

100**

VvPNRLin

(3R)-Linalool syn

HM807390

PNTPS09M1

VvTPS31

(3R)-linalool

100

  1. Terpenoids produced by individual VvTPS clones when incubated with GPP are listed. Where noted are bifunctional TPS capable of producing products when incubated with GPP (*) or FPP (**). The tissue specific cDNA used to clone a particular gene is indicated in FLcDNA ID with either B (berry), YB (young berry), F (flower), S (stem) or M (mixed template consisting of stems, berries, flowers and leaves). FLcDNA ID in bold indicates clone came from cDNA of methyl jasmonate treated tissue. Clones with redundant functions are listed as well. Bolded VvTPS gene models indicate proof of transcript not previously known from the available ESTs.