Table 4 Genes selected for Real-time RT-PCR.
Gene | Description | Forward primer | Reverse primer | Target size | Solexa fold | RT-PCR fold |
|---|---|---|---|---|---|---|
CHI4D | V. vinifera class IV chitinase (gb|AF532966.1) | TCCCACGTTCCCCCTTCT | GTAGCTTGGCTGCCATTTTTG | 59 | 11 | 4 |
TL3 | V.vinifera thaumatin-like protein (gb|AF532965.1) | ACCCCACTCCAACCATCAAG | GATTTTGCAGAGGCCCATTG | 59 | 57 | 4 |
PR10 | Tamnara Tam-RP10 pathogenesis-related protein 10 (dbj|AB372561.1) | GGTCAGGCCTCAAGCTATCAA | CAGGGCCTCCGTCTCCTT | 56 | 10 | 3 |
TIP2;1 | V. vinifera aquaporin TIP2;1 (gb|EF364439.1) | GCATCATTGCACCCATTGC | GCCTGCAGCCAGGATGTT | 59 | 6 | 1 |
CYSP | V. vinifera cysteine protease (gb|EU280160.1) | CCTCGCAGGAGGAGCACGAT | CCGGCGCAGGTTTGC | 54 | 2 | 1 |
ERF4 | V. aestivalis putative ethylene response factor 4 (gb|AY484580.1) | TCATCACTGCAACTCATCCA | TTACAATCTTCGGCCTCTGA | 101 | 11 | 4 |
STS5 | V. vinifera stilbene synthase5 (gb|AY670312.1) | CGCTCAAGGGAGGAAAGACA | AGCCAAACAAAACACCCCAATC | 58 | 12 | 18 |
THX | thioredoxin x [Populus trichocarpa] (XP_002310066.1) | TGCTCAGGAATACGGGGACAGA | TCGCGGGTTTGCATCAT | 61 | -39 | -2 |
SHM1 | A. thaliana serine hydroxymethyl transferase 1 (ref|NM_119954.3) | TGTTCATCAGGTCAGCCAGTTT | TGCGTCGAATTGCAGCAAGAT | 63 | -2 | -2 |
HypP | Hypothetical protein LOC100264849 | TGCCCCTACCCTTGTGACA | GATCAAAATGGCTCATCGGAA | 58 | -5 | -3 |
GLO | V. pseudoreticulata glyoxal oxidase (gb|D201181.1) | TCCCAACGCCGGTATAGC | ACCGTGCCGTAACGTGTGA | 54 | -5 | -1 |
ClpP | Carica papaya ATP-dependent Clp protease proteolytic subunit (gb|DQ159405.1|) | GGGCGCCGGACAAGA | TTTGCAAATCATCCCTAATGGA | 55 | -2 | -2 |