The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

Table 1 Media composition for the wheat and barley tissue and suspension cultures.

Component	Medium B5	Medium HV
	mg/l	mg/l
NaH₂PO₄ × H₂O	172
KH₂PO₄		34
CaCl₂ × 2 H₂O	150	88
(NH₄)₂SO₄	134
NH₄NO₃		1650
MgSO₄ × 7 H₂O	250	370
KNO₃	2500	1900
FeSO₄ × 7 H₂O	25.6	11.12
Na₂EDTA × 2 H₂O	34.27	15.72
KJ	0.75	0.166
MnSO₄ × H₂O	10	4.46
H3BO₃	3	1.24
ZnSO₄ × 7 H₂O	3	1.72
Na₂MoO₄ × 2 H₂O	0.25	0.05
CuSO₄ × 5 H₂O	0.25	0.005
CoCl₂ × 6 H₂O	0.25	0.005
Nicotinic acid	1	1
Thiamine hydrochloride	10	10
Pyridoxal hydrochloride	1	1
myo-Inositol	100	100
2,4-Dichlorophenoxyacetic acid	2	2
Sucrose	20000	30000
(Agar)	(8000)	(8000)
	pH = 5.5	pH = 6.0

ISSN: 1471-2229