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Table 1 Gene bank accession numbers and primer sequences used in real-time PCR of differentially expressed ESTs

From: Identification of genes associated with growth cessation and bud dormancy entrance using a dormancy-incapable tree mutant

Gene name EST bank accession #   Forward (F) and reverse (R) primer sequence
Desiccation-related protein, putative GE653173 F 5'-AGGGCTCGACGATATCAGTCC-3'
   R 5'-TGCATACGGGTCAAATGCAGG-3'
Amidase family protein GE653170 F 5'-CGGTTCGATCCTTGGCAGAC-3'
   R 5'-TCGACACGGCTGCAAATGGAG-3'
Deoxynucleoside kinase family protein GE653171 F 5'-AGGAGGACAGCTCGAACTCAG-3'
   R 5'-GCATACCTCTGCGGCTCAGC-3'
Auxin-binding protein ABP20 precursor GE653207 F 5'-AGCCTCACCTCCATTGACTTGG-3'
   R 5'-TGTTGCTCAGTTTCCTGGTGTGA-3'
Amino acid transporter family protein GE653321 F 5'-GGCTTCACCCATGACATCACC-3'
   R 5'-CTGGAATTATGAGCCTGCCTGC-3'
Glycoside hydrolase, family 18 GE653328 F 5'-CAGTCCACCACTCCCATCACTG-3'
   R 5'-GCTTCCATTGCTCCCTTCGATG-3'
ATP sulfurylase 1 GE653245 F 5'-ACAAGACGCAATGCTGATGCTG-3'
   R 5'-ACCACAGTCGTCTCAGGATCAAG-3'
KEG (KEEP ON GOING) protein GE653332 F 5'-ACCCGTTCTATTTCCGATGCCT-3'
   R 5'-TCAGTTTCAACTCCAACCCACCA-3'
Phosphatidylinositol 3- and 4-kinase family protein GE653311 F 5'-GGGTTGGGGAGACAGGTTTCA-3'
   R 5'-AGTCCCATGATCACTGGCATCA-3'
PRH75 (DEAD-box helicase) GE653257 F 5'-TGTAGCCAGCAGCCTTAGCAAG-3'
   R 5'-GCCAGTTGATGTTGCCAAAGCAG-3'
Zinc ion binding/LIM GE653319 F 5'-AGAAGAGGATGAGGAGGAAGACG-3'
   R 5'-GTTGTTGACAGGGTCGATTCTGG-3'
ATP-binding cassette transporter MRP6 GE653330 F 5'-CTGGGATTGTGGGTAGAACTGG-3'
   R 5'-CCTTCAAACATGGTTGGGTCCTG-3'
Unknown1 GE653303 F 5'-CTCTCTGTGCTTCTCTCTCCTCA-3'
   R 5'-TCCAGATTAACTCAGGGAGAAACCAG-3'
Late embryogenesis abundant (LEA) GE653244 F 5'-TTCAAATTCTCCGGGGGTCG-3'
   R 5'-TTCCAGGCCATCTTCCACGG-3'
Metallothionein-like protein GE653329 F 5'-TCCACCAATCAACAAACACCTCAC-3'
   R 5'-TAGCAAGTAATCTATGCGTGTGTGG-3'
Pathogenesis-related protein 1a (PR-1a) GE653248 F 5'-CGACTGCAATCTTGTCCACTCTGG-3'
   R 5'-ACCTCCACTGTTGCACCTCAC-3'
Dormancy associated MADS-box gene 1 (PpDAM1) GE653327 F 5'-CAGAGGGCAAGCAACTACCAC-3'
   R 5'-CCAGAGAAATTATGGAAGCCCCA-3'
Dormancy associated MADS-box gene 6 (PpDAM6) GE653238 F 5'-CCAACAACCAGTTAAGGCAGAAGA-3'
   R 5'-GGAAGCCCCAGTTTGAGAGA-3'
Epicotyl-specific tissue protein GE653203 F 5'-CACCAAAAGAGAAAGCCGACTGC-3'
   R 5'-TCAACCTCAACGTCAACCTCAAC-3'
RD22 (dehydration-responsive) precursor GE653312 F 5'-GAACCCACACAAGATTATCAGCAGG-3'
   R 5'-TTCTACTGCCACAGCCAGCA-3'
Unknown2 GE653334 F 5'-ATGGCAAACCACCAAGCACTCA-3'
   R 5'-GTAGAGGAGCCTTGATTGGAGGAG-3'
Unknown3 GE653309 F 5'-AAGTTGTCCATCCCAACACATTCG-3'
   R 5'-GCGAGGACATCTCTGGCAATAAGA-3'
Unknown4 GE653307 F 5'-TCCTCAACGAACAGACGGAACTC-3'
   R 5'-TGGTGGTCTTGGAAATGCTGGT-3'