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Table 1 Assembly statistics of non-normalized and normalized cDNA libraries.

From: Mining the bitter melon (momordica charantial.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes

 

Non-normalized

Normalized

Number of sequences

404,468

255,687

Number of high quality sequences

281,933

216,950

Number of the clean reads

228,000

177,991

Number of singlets

53,897

38,959

 

(23.6%)

(21.8%)

Number of contigs

10,072

18,245

Average length of contigs

297

414

Average length of EST

828

796

Average read of contigs

23

10

Number of contigs match Viridiplants

4,459

11,558

Number of contigs match Arabidopsis

3,643

11,211

(# of unique homologs in Arabidopsis)

(2,187)

(6,737)

Lipid genes contigs

193

345

(# of unique homologs for known Arabidopsis lipid genes)

(85)

(189)