Table 1 Conservation of key residues for FK506 binding/PPIase activity in O. sativa FKBPs (OsFKBPs) and comparison with those of A. thaliana FKBPs.
OsFKBPs/AtFKBPs | Residues for FK506 binding/PPIase activity in human FKBP12 (aa position) | Conserveda (%) | Similarityb (%) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | 27 | 37 | 38 | 43 | 47 | 55 | 56 | 57 | 60 | 82 | 83 | 88 | 92 | 100 | Â | Â |
hFKBP12 | Y | F | D | R | F | E | V | I | W | A | Y | H | I | F | Â | Â | |
OsFKBP12 | : AtFKBP12 | C | " | W | A/E | " | S/A | " | " | " | " | " | F | " | " | 64/64 | 86 |
OsFKBP13 | : AtFKBP13 | " | " | " | " | L | " | " | " | " | " | " | A | " | " | 86/86 | 100 |
OsFKBP15-1 | : AtFKBP15-1 | " | " | " | " | I | Q | " | " | " | G | " | S | " | " | 71/71 | 100 |
OsFKBP15-2 | : AtFKBP15-2 | " | " | " | " | F | Q | " | " | " | G | " | S | " | " | 71/71 | 100 |
| Â | : AtFKBP15-3 | " | " | " | K | Y | K | " | " | L | G | " | - | " | " | -/57 | Â |
OsFKBP16-1 | : AtFKBP16-1 | " | V | H | Q | V | "/D | M/V | " | L | G | " | P | L | " | 36/29 | 86 |
OsFKBP16-2 | : AtFKBP16-2 | " | " | " | " | L | K | I/" | L/" | L | " | " | G | " | Y | 50/64 | 86 |
OsFKBP16-3 | : AtFKBP16-3 | " | " | " | K | Y | Q | " | " | L | " | F | A | V | " | 50/50 | 100 |
OsFKBP16-4 | : AtFKBP16-4 | " | " | M | G | Y | " | " | L | L | " | " | V | A/L | " | 50/50 | 93 |
OsFKBP17-1 | : AtFKBP17-1 | " | " | " | H | " | N/K | " | " | I | G | " | Q | P | " | 57/57 | 93 |
OsFKBP17-2 | : AtFKBP17-2 | L | V | V | G/K | L | P | Y | T/S | M/L | G | F | A | V/" | Y | 0/7 | 71 |
| Â | : AtFKBP17-3 | V | V | " | K | L | P | Y | S | L | G | F | Q | " | Y | -/14 | Â |
OsFKBP18 | : AtFKBP18 | F | L | A | I | Y | P | G | K | K | G | " | M | " | L | 14/14 | 100 |
OsFKBP19 | : AtFKBP19 | W | " | E | D | " | Q/" | " | " | F | G | " | Y | G | " | 43/50 | 93 |
OsFKBP20-1a | : AtFKBP20-1 | " | " | " | D | " | A/S | " | " | " | " | " | S | " | " | 79/79 | 93 |
OsFKBP20-1b | Â | " | " | " | D | " | T | " | " | " | " | " | S | " | " | 7/- | Â |
OsFKBP20-2 | : AtFKBP20-2 | " | I | " | Q | I | T/A | L | V | F | G | P | P | F | " | 21/21 | 93 |
OsFKBP42a | : AtFKBP42 | " | " | E | E | I | K | E | M/L | L | G/" | " | S | V | Y | 21/29 | 86 |
OsFKBP42b | Â | " | " | E | E | I | K | Q | M | L | G | " | S | V | Y | 21/29 | Â |
| Â | : AtFKBP43 | " | " | " | E | L | N | " | " | L | G | " | G | K | Y | -/43 | Â |
OsFKBP44 | Â | " | V | " | S | C | " | " | " | " | G | " | R | " | " | 64/- | Â |
OsFKBP46 | Â | F | " | V | N | " | N | " | M | L | " | I | S | " | " | 43/- | Â |
OsFKBP53 | : AtFKBP53 | " | V/" | E/" | E/K | " | K/S | " | " | " | C/G | " | I/A | V/" | Y/" | 43/64 | 44 |
OsFKBP57 | Â | " | V | S | - | I | F | F | C | F | G | F | R | V | V | 7/- | Â |
OsFKBP58 | Â | " | " | " | " | " | " | " | " | " | G | " | R | " | " | 79/- | Â |
OsFKBP59 | Â | " | I | " | N | H | " | " | " | " | G | " | T | " | Y | 57/- | Â |
OsFKBP62a | : AtFKBP62 | " | " | " | " | " | Q | " | " | " | " | " | S | "/- | " | 86/79 | 93 |
OsFKBP62b | : AtFKBP62 | " | " | " | " | " | "/Q | " | " | " | " | " | S | "/- | " | 93/79 | 86 |
OsFKBP62c | : AtFKBP62 | " | " | " | " | " | Q | " | " | " | " | " | S | "/- | " | 86/79 | 93 |
| Â | : AtFKBP65 | " | " | " | " | " | H | " | " | " | " | " | S | - | " | -/79 | Â |
OsFKBP72 | : AtFKBP72 | " | Y | " | N | " | L | " | P | F | " | " | R | V | W | 43/43 | 100 |
OsFKBP73 | Â | F | " | V | N | E | D | " | M | F | T | M | S | " | " | 36/- | Â |
Conservedc (%) | 79/77 | 68/73 | 57/64 | 32/27 | 43/36 | 25/14 | 75/73 | 68/68 | 46/36 | 46/46 | 82/82 | 0/0 | 46/50 | 64/64 | Â | Â | |