Figure 5From: Comparative genomic analysis of 1047 completely sequenced cDNAs from an Arabidopsis-related model halophyte, Thellungiella halophilaEffect of salt stress on transcript levels of ThSOS1 and ThSOS1S. (A) Schematic of the corresponding regions of the primers in ThSOS1, ThSOS1S and AtSOS1 for RT-PCR analysis. Exons are indicated as filled boxes and introns as spaces between them. (B) Relative expression of ThSOS1 and ThSOS1S under salt stress. Two-week-old Thellungiella seedlings were exposed to 250 mM NaCl stress for 0, 5, or 10 h. Total RNA was reversed-transcribed into cDNA and used as a template for quantitative RT-PCR. The qRT-PCR performed using blue-primer sets in (A). Relative transcript levels were normalized to Actin2 mRNA. Data are the mean ± SD for three individual experiments (n = 3). (C) Semiquantitative gene expression of SOS1 splice variants in Thellungiella and Arabidopsis under normal growth conditions. The RT-PCR performed using red-primer sets in (A), which were able to detect these splice variants.Back to article page