Figure 3From: A high-throughput method for the detection of homoeologous gene deletions in hexaploid wheatAmplification profiles of TaPFT1 homoeologues in the wheat genotype Chara. Fluorescence generated from a multiplexed-assay using the TaPFT1 PCR primers and homoeologue-specific probes shown in Figure 2A using cv. Chara genomic DNA as a template. A strong increase in fluorescence was detected in the three specific channels each detecting a different fluorescent signal (FAM [curve 1], NED [curve 2], and VIC [curve 3]), from the amplification of TaPFT1 homoeologous sequences with CT values of approximately 25 for all probes. Data generated in an ABI 7900HT as an absolute quantitation amplification plot. Note that Y axis is a logarithmic scale.Back to article page