Location of mlo mutant sites within the predicted consensus secondary structure of the Mlo second and third cytoplasmic loop. Consensus secondary structures of the second and third cytoplasmic loop regions of monocot and dicot Mlo orthologs were calculated with the JPRED web tool (see Methods for details). Amino acids are given in the single letter code ("OrigSeq") and the final predicted secondary structure("Jnet") is indicated by a red H (α helix), a yellow E (β sheet) or dashes (random coil) below the sequence. The color code (highlighted residues) indicates amino acids that lead to non-functional (<50% entry rate compared to wild-type Mlo) mutant variants with wild type-like accumulation (green), intermediate accumulation (yellow), or severely affected accumulation (red). "hmm" indicates the Jnet hidden Markov model prediction; "Jnet_25", "Jnet_5" and "Jnet_0" signify the predicted relative solvent accessibility using 25%, 5% and 0% cut-off values. The prediction indicates whether a residue is buried ("B") or exposed ("-") at each of the three relative solvent accessibility cut-offs. "Jnet Rel" specifies the prediction accuracy for each position, ranging from 0 to 9 (the higher the better).