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Table 1 Purification table.

From: STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro

Purification Step

K2D50

%

Error

Purification

Factor

Exudate

16.38

0.4

1.0

Chloroform supernatant

9.78

21.8

1.7

1st MonoQ*

7.35 × 10-04

22.9

22,276

C18*

1.28 × 10-03

18.6

12,769

2nd MonoQ

1.04 × 10-04

+

156,849

  1. (*) No significant difference in a t-test (p > 0.05).
  2. (+) No duplicates available.
  3. K2D50 represents Abs280 units necessary for 50% dephosphorylation of LePRK2; values were obtained from an average of two independent purifications. % Error is calculated as (Standard error/K2D50) × 100. The purification factor was calculated by comparison to the starting exudate.
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BMC Plant Biology

ISSN: 1471-2229

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