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Table 3 PCR amplification conditions

From: Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

Primer pair

(upper primer lower primer)

purpose

Annealing temperature

Extension time

F24D7-9radR

F24D7-9radF

Screening of BAC library

55.5°C

1 min

F24D7-13radR

F24D7-13radF

Screening of BAC library

54°C

1 min

PPRA:20505U22

PPRA:20954L21

RT-PCR on PPR-A and PPR-2

52°C

30 sec

Rfocons1047U22

PPRB:13225L22

RT-PCR on PPR-B

52°C

1 min 15 sec

PPR1:21229U22

PPRB:13225L22

RT-PCR on PPR-1

51°C

1 min