Low-magnification CLSM detection of superoxide anion (O
.-) with DHE at different developmental stages of the olive flower. Projections of section stacks A: the presence of O2 .- is shown by green fluorescence, which is clearly distinguishable from the tissues autofluorescence (red colour). Two different treatments are displayed (DHE alone or in combination with TMP), as well as untreated samples (control). B: quantification of the fluorescence intensity owing to DHE under the different treatments over the stigma surface. C: the same over the anther surface. Both the average and the standard deviation displayed in the graphs correspond to the measurement of a minimum of nine images, on three independent experiments. A: anther; AU: arbitrary units; O: ovary; S: stigma; St: style.