Complementation of the xyn11A mutation in B. cinerea by the exogenous addition (by infiltration) of wild-type or non-xylan-hydrolyzing mutant Xyn11A proteins. The indicated proteins were infiltrated in tomato leaves 4 hours before inoculation with conidia of the indicated strains. A) Infection areas generated by the wild-type strain B05.10 and the xyn11A mutant N23, inoculated on spots previously infiltrated with wild-type (wt) or mutant (E214S) Xyn11A (X) proteins. Mean areas were calculated from at least 5 infections. B) Example leaf of the experiment in (A). Bars marked with different letters are statistically different (P < 0.05 by Student's t test).