Table 2 Optimal genes for quantification of individual and mixed organs in each Petunia line.
Statistic | Flower | Leaf | ||||||
|---|---|---|---|---|---|---|---|---|
Mitchell | ||||||||
| Â | A | B | C | D | A+B+C+D | A | C | A+C |
M geNorm | EF1α (0.05) RPS13 (0.05) | SAND (0.14) UBQ (0.14) | EF1α (0.13) RPS13 (0.13) | RAN1 (0.08) RPS13 (0.08) | RAN1 (0.47) SAND (0.47) | EF1α (0.11) RPS13 (0.11) | RAN1 (0.14) SAND (0.14) | EF1α (0.37) RPS13 (0.37) |
M qBasePlus | ACT (0.55) RPS13 (0.56) | EF1α (0.60) RPS13 (0.60) | CYP (0.60) SAND (0.64) | EF1α (0.30) RAN1 (0.34) | EF1α (0.80) SAND (0.82) | RPS13 (0.77) EF1α (0.78) | SAND (0.93) RAN1 (0.93) | EF1α (0.85) RAN1 (0.92) |
CV qBasePlus | ACT (0.05) SAND (0.15) | RPS13 (0.07) CYP (0.25) | CYP (0.09) SAND (0.11) | EF1α (0.05) TUB (0.14) | EF1α (0.25) SAND (0.28) | RPS13 (0.14) RAN1 (0.18) | SAND (0.12) RAN1 (0.18) | RAN1 (0.20) EF1α (0.21) |
Min. number | 2 (0.04) | 2 (0.07) | 2 (0.12) | 2 (0.05) | 4 (0.15) | 2 (0.10) | 2 (0.13) | 2 (0.12) |
V30 | ||||||||
Statistic | Flower | Leaf | ||||||
| Â | A | B | C | D | A+B+C+D | A | C | A+C |
M geNorm | RAN1 (0.11) UBQ (0.11) | TUB (0.12) CYP (0.12) | RPS13 (0.23) UBQ (0.23) | ACT (0.02) CYP (0.02) | RAN1 (0.45) ACT (0.45) | TUB (0.07) RAN1 (0.07) | RPS13 (0.09) TUB (0.09) | RAN1 (0.20) UBQ (0.20) |
M qBasePlus | CYP (0.30) RAN1 (0.33) | CYP (0.66) TUB (0.69) | SAND (0.63) CYP (0.63) | RPS13 (0.29) EF1α (0.30) | ACT (2.27) RAN1 (2.44) | TUB (0.34) UBQ (0.36) | SAND (0.27) RPS13 (0.29) | UBQ (0.49) RPS13 (0.51) |
CV qBasePlus | CYP (0.05) TUB (0.10) | RAN1 (0.09) CYP (0.11) | SAND (0.10) ACT (0.25) | EF1α (0.06) CYP (0.06) | ACT (0.70) RAN1 (0.82) | UBQ (0.06) TUB (0.09) | SAND (0.03) RPS13 (0.06) | UBQ (0.14) RPS13 (0.16) |
Min. number | 2 (0.10) | 2 (0.07) | 2 (0.09) | 2 (0.04) | NA | 2 (0.04) | 2 (0.03) | 2 (0.07) |