Table 1 Reference genes and their primer sequences that were selected for evaluation of expression stability during flower development in cotton (Gossypium hirsutum) for qPCR analysis, as the sequence of two genes of interest MADS-box.
Gene abbreviation | Acession | A. thaliana ortholog locus | A. thaliana annotation | Similarity (e-value) | Identity (%) | Gene Size ** | Blast alignment | Primer sequence |
|---|---|---|---|---|---|---|---|---|
GhACT4 | AY305726 | At5g09810 | Actin gene family | 6.90E-194 | 86% | 1700 | 1013 | TTGCAGACCGTATGAGCAAG/ATCCTCCGATCCAGACACTG |
GhEF1α 5 | DQ174254 | At5g60390 | Elongation Factor 1-alpha | 5.30E-225 | 85% | 1764 | 1193 | TCCCCATCTCTGGTTTTGAG/CTTGGGCTCATTGATCTGGT |
*GhFBX6 | DR463903 | At5g15710 | F-box family protein | 2.30E-93 | 79% | 1884 | 567 | TGCCTGCAGTAAATCTGTGC/GGGTGAAAGGGTTTCCAAAT |
*GhPP2A1 | DT545658 | At1g59830 | Catalytic subunit of protein phosphatase 2A | 3.30E-110 | 77% | 1301 | 675 | GATCCTTGTGGAGGAGTGGA/GCGAAACAGTTCGACGAGAT |
*GhMZA | DT571956 | At5g46630 | Clathrin adaptor complexes medium subunit family protein | 1.40E-131 | 82% | 1853 | 755 | CCGTCAGACAGATTGGAGGT/AAAGCAACAGCCTCAACGAC |
*GhPTB | DT574577 | At3g01150 | Polypyrimidine tract-binding protein homolog | 1.50E-120 | 77% | 1511 | 752 | GGTTACCATTGAGGGTGTGG/GTGCACAAAACCAAATGCAG |
*GhGAPC2 | ES810306 | At1g13440 | Glyceraldehyde-3-phosphate dehydrogenase C-2 | 0.0 | 83% | 1439 | 858 | TCCCCATCTCTGGTTTTGAG/AACCCCATTCGTTGTCCATA |
GhβTUB3 | AY345606 | At5g12250 | Beta-tubulin | 5.70E-198 | 80% | 1696 | 1135 | GATTCCCTTCCCTCGTCTTC/CGGTTAGAGCTCGGTACTGC |
***GhUBQ14 | DW505546 | At4g02890 | Polyubiquitin | 0.0 | 80% | 1502 | 510 | CAACGCTCCATCTTGTCCTT/TGATCGTCTTTCCCGTAAGC |
GhMADS3 | ES812912 | At4G18960 | AGAMOUS | NA | NA | NA | NA | ATCAAGCGGATCGAAAACAC/CAACCTCAGCGTCACAAAGA |
GhSEP-like1 | ES827315 | At1G24260 | SEPALLATA3 | NA | NA | NA | NA | TCCGTTCTTTGTGATGCAGA/CCATGGCTGCACTTCTGGTA |