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Table 2 Values of efficiency ± standard deviation (SD) of the primers of the housekeeping genes and average values of quantification cycle (Cq) ± standard deviation (SD) of biological replicates generated by the Miner to the genes of reference of G. hirsutum.

From: Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

A GhACT4 GhEF1α 5 GhFBX6 GhPP2A1 GhMZA GhPTB GhGAPC2 GhβTUB3 GhUBQ14
Efficiency ± SD 0.93 ± 0.026 0.97 ± 0.019 0.93 ± 0.018 0.91 ± 0.019 0.91 ± 0.021 0.93 ± 0.014 0.89 ± 0.031 0.94 ± 0.015 0.93 ± 0.022
Plant organs Cq ± SD
Leave 19.08 ± 0.395 19.20 ± 0.705 24.74 ± 0.191 23.66 ± 0.442 21.45 ± 1.388 23.40 ± 0.940 24.57 ± 0.663 22.29 ± 0.084 18.57 ± 0.333
Stem 17.45 ± 0.199 17.39 ± 0.150 24.99 ± 0.251 22.36 ± 0.290 21.15 ± 0.216 22.49 ± 1.592 21.65 ± 0.980 19.39 ± 0.323 16.36 ± 0.201
Branch 17.74 ± 0.648 17.25 ± 0.157 24.16 ± 0.026 22.38 ± 0.268 21.58 ± 0.092 22.20 ± 0.614 23.38 ± 0.642 19.26 ± 0.072 16.63 ± 0.187
Root 17.46 ± 0.337 18.05 ± 0.107 24.54 ± 0.991 23.06 ± 0.655 22.72 ± 0.233 22.33 ± 0.377 25.28 ± 0.236 22.45 ± 0.292 18.32 ± 0.561
Flower buds 16.70 ± 0.262 16.80 ± 0.493 23.77 ± 0.042 22.63 ± 0.141 21.71 ± 0.451 22.51 ± 1.088 24.09 ± 0.936 21.73 ± 0.174 18.20 ± 0.323
Fruits 16.25 ± 0.273 16.71 ± 0.188 24.07 ± 0.712 22.60 ± 0.181 21.46 ± 0.240 22.69 ± 0.241 24.18 ± 0.160 19.17 ± 0.135 16.51 ± 0.193
B GhACT4 GhEF1α 5 GhFBX6 GhPP2A1 GhMZA GhPTB GhGAPC2 GhβTUB3 GhUBQ14
Efficiency ± SD 0.96 ± 0.015 0.95 ± 0.014 0.94 ± 0.015 0.92 ± 0.017 0.94 ± 0.020 0.93 ± 0.022 0.88 ± 0.024 0.94 ± 0.017 0.94 ± 0.013
Flower buds Cq ± SD
Floral meristem 16.84 ± 0.34 16.14 ± 0.57 23.76 ± 0.44 21.78 ± 0.73 20.94 ± 0.39 21.60 ± 0.33 24.98 ± 0.26 19.89 ± 0.32 17.31 ± 0.78
Flower bud 2 mm 20.61 ± 1.78 24.70 ± 1.59 27.93 ± 1.34 25.37 ± 1.90 25.52 ± 3.07 27.26 ± 2.27 28.49 ± 2.41 24.70 ± 1.59 21.16 ± 1.85
Flower bud 4 mm 18.53 ± 0.92 23.49 ± 0.96 25.62 ± 1.32 24.24 ± 1.11 21.94 ± 0.08 23.97 ± 1.54 27.60 ± 0.84 23.49 ± 0.96 19.04 ± 1.30
Flower bud 6 mm 15.76 ± 0.14 20.37 ± 0.24 23.41 ± 0.10 22.01 ± 0.10 20.81 ± 0.14 21.65 ± 0.21 21.03 ± 0.64 20.37 ± 0.24 16.23 ± 0.51
Flower bud 7 mm 17.17 ± 1.19 20.90 ± 0.99 24.22 ± 1.26 22.47 ± 1.10 22.55 ± 0.56 22.47 ± 0.91 21.69 ± 1.26 20.90 ± 0.99 16.99 ± 1.08
Flower bud 8 mm 16.44 ± 0.74 20.54 ± 0.18 24.34 ± 0.66 22.09 ± 0.84 21.07 ± 1.21 22.64 ± 0.78 20.98 ± 0.49 20.54 ± 0.18 16.70 ± 0.38
Flower bud 10 mm 18.06 ± 0.71 22.01 ± 1.45 26.09 ± 0.16 23.56 ± 1.54 21.68 ± 1.20 23.36 ± 0.89 22.04 ± 1.76 22.01 ± 1.45 17.38 ± 1.15
Flower bud 12 mm 15.30 ± 0.64 19.33 ± 0.83 24.03 ± 0.52 21.69 ± 0.13 20.03 ± 0.65 21.54 ± 0.62 21.41 ± 0.96 19.51 ± 0.77 15.98 ± 0.45
C GhACT4 GhEF1α 5 GhFBX6 GhPP2A1 GhMZA GhPTB GhGAPC2 GhβTUB3 GhUBQ14
Efficiency ± SD 0.97 ± 0.021 0.92 ± 0.029 0.94 ± 0.017 0.82 ± 0.019 0.92 ± 0.024 0.91 ± 0.031 0.88 ± 0.032 0.93 ± 0.009 0.96 ± 0.024
Floral organs Cq ± SD
Carpels 17.34 ± 0.52 17.16 ± 1.18 24.11 ± 0.73 22.31 ± 0.66 20.85 ± 0.40 21.93 ± 0.77 22.14 ± 1.60 21.20 ± 0.28 16.12 ± 0.63
Stames 16.87 ± 0.29 16.08 ± 0.19 24.37 ± 0.09 22.12 ± 0.59 21.59 ± 0.31 21.78 ± 0.70 22.76 ± 0.53 21.33 ± 0.20 17.77 ± 0.29
Sepals 16.33 ± 0.39 15.82 ± 0.63 23.08 ± 0.36 21.96 ± 0.47 20.66 ± 0.19 21.50 ± 0.18 23.24 ± 0.12 20.31 ± 0.20 16.17 ± 0.85
Petals 18.08 ± 2.00 18.55 ± 2.52 25.39 ± 1.37 23.17 ± 0.79 22.65 ± 1.72 23.51 ± 1.56 24.09 ± 0.13 21.25 ± 1.93 18.51 ± 1.99
Pedicels 16.56 ± 0.19 16.11 ± 0.32 25.02 ± 0.85 23.69 ± 0.11 22.52 ± 0.92 23.28 ± 0.72 22.25 ± 0.56 21.60 ± 0.08 16.28 ± 0.33
D GhACT4 GhEF1α 5 GhFBX6 GhPP2A1 GhMZA GhPTB GhGAPC2 GhβTUB3 GhUBQ14
Efficiency ± SD 0.96 ± 0.019 0.94 ± 0.17 1.01 ± 0.012 0.94 ± 0.017 1.01 ± 0.018 0.98 ± 0.014 0.96 ± 0.018 0.94 ± 0.026 0.93 ± 0.020
Fruits Cq ± SD         
Fruits 10-15 mm 16.78 ± 0.74 18.56 ± 1.36 26.33 ± 0.30 23.43 ± 1.00 22.44 ± 0.65 24.13 ± 0.57 27.85 ± 0.51 20.67 ± 0.27 17.52 ± 0.15
Fruits 16-20 mm 17.27 ± 0.19 18.49 ± 1.17 26.64 ± 0.93 22.78 ± 1.10 20.89 ± 0.07 23.12 ± 0.48 26.79 ± 0.70 19.61 ± 0.42 17.28 ± 0.26
Fruits 21-30 mm 17.39 ± 0.47 18.89 ± 0.14 26.09 ± 0.75 23.34 ± 0.21 21.45 ± 0.28 22.75 ± 0.98 27.39 ± 0.67 20.14 ± 1.30 17.17 ± 0.18
Fruits >30 mm 19.89 ± 1.58 20.89 ± 1.78 29.17 ± 2.12 24.61 ± 0.72 23.06 ± 0.72 24.70 ± 0.46 26.94 ± 2.49 20.64 ± 1.37 18.70 ± 1.15
  1. The values of efficiency of primers were generated for each experimental situation (A-plant organs, B-flower buds, C-floral organs and D-fruit).