Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

Artico, Sinara; Nardeli, Sarah M; Brilhante, Osmundo; Grossi-de-Sa, Maria Fátima; Alves-Ferreira, Marcio

doi:10.1186/1471-2229-10-49

Table 2 Values of efficiency ± standard deviation (SD) of the primers of the housekeeping genes and average values of quantification cycle (Cq) ± standard deviation (SD) of biological replicates generated by the Miner to the genes of reference of G. hirsutum.

From: Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

A	GhACT4	GhEF1α 5	GhFBX6	GhPP2A1	GhMZA	GhPTB	GhGAPC2	GhβTUB3	GhUBQ14
Efficiency ± SD	0.93 ± 0.026	0.97 ± 0.019	0.93 ± 0.018	0.91 ± 0.019	0.91 ± 0.021	0.93 ± 0.014	0.89 ± 0.031	0.94 ± 0.015	0.93 ± 0.022
Plant organs	Cq ± SD
Leave	19.08 ± 0.395	19.20 ± 0.705	24.74 ± 0.191	23.66 ± 0.442	21.45 ± 1.388	23.40 ± 0.940	24.57 ± 0.663	22.29 ± 0.084	18.57 ± 0.333
Stem	17.45 ± 0.199	17.39 ± 0.150	24.99 ± 0.251	22.36 ± 0.290	21.15 ± 0.216	22.49 ± 1.592	21.65 ± 0.980	19.39 ± 0.323	16.36 ± 0.201
Branch	17.74 ± 0.648	17.25 ± 0.157	24.16 ± 0.026	22.38 ± 0.268	21.58 ± 0.092	22.20 ± 0.614	23.38 ± 0.642	19.26 ± 0.072	16.63 ± 0.187
Root	17.46 ± 0.337	18.05 ± 0.107	24.54 ± 0.991	23.06 ± 0.655	22.72 ± 0.233	22.33 ± 0.377	25.28 ± 0.236	22.45 ± 0.292	18.32 ± 0.561
Flower buds	16.70 ± 0.262	16.80 ± 0.493	23.77 ± 0.042	22.63 ± 0.141	21.71 ± 0.451	22.51 ± 1.088	24.09 ± 0.936	21.73 ± 0.174	18.20 ± 0.323
Fruits	16.25 ± 0.273	16.71 ± 0.188	24.07 ± 0.712	22.60 ± 0.181	21.46 ± 0.240	22.69 ± 0.241	24.18 ± 0.160	19.17 ± 0.135	16.51 ± 0.193
B	GhACT4	GhEF1α 5	GhFBX6	GhPP2A1	GhMZA	GhPTB	GhGAPC2	GhβTUB3	GhUBQ14
Efficiency ± SD	0.96 ± 0.015	0.95 ± 0.014	0.94 ± 0.015	0.92 ± 0.017	0.94 ± 0.020	0.93 ± 0.022	0.88 ± 0.024	0.94 ± 0.017	0.94 ± 0.013
Flower buds	Cq ± SD
Floral meristem	16.84 ± 0.34	16.14 ± 0.57	23.76 ± 0.44	21.78 ± 0.73	20.94 ± 0.39	21.60 ± 0.33	24.98 ± 0.26	19.89 ± 0.32	17.31 ± 0.78
Flower bud 2 mm	20.61 ± 1.78	24.70 ± 1.59	27.93 ± 1.34	25.37 ± 1.90	25.52 ± 3.07	27.26 ± 2.27	28.49 ± 2.41	24.70 ± 1.59	21.16 ± 1.85
Flower bud 4 mm	18.53 ± 0.92	23.49 ± 0.96	25.62 ± 1.32	24.24 ± 1.11	21.94 ± 0.08	23.97 ± 1.54	27.60 ± 0.84	23.49 ± 0.96	19.04 ± 1.30
Flower bud 6 mm	15.76 ± 0.14	20.37 ± 0.24	23.41 ± 0.10	22.01 ± 0.10	20.81 ± 0.14	21.65 ± 0.21	21.03 ± 0.64	20.37 ± 0.24	16.23 ± 0.51
Flower bud 7 mm	17.17 ± 1.19	20.90 ± 0.99	24.22 ± 1.26	22.47 ± 1.10	22.55 ± 0.56	22.47 ± 0.91	21.69 ± 1.26	20.90 ± 0.99	16.99 ± 1.08
Flower bud 8 mm	16.44 ± 0.74	20.54 ± 0.18	24.34 ± 0.66	22.09 ± 0.84	21.07 ± 1.21	22.64 ± 0.78	20.98 ± 0.49	20.54 ± 0.18	16.70 ± 0.38
Flower bud 10 mm	18.06 ± 0.71	22.01 ± 1.45	26.09 ± 0.16	23.56 ± 1.54	21.68 ± 1.20	23.36 ± 0.89	22.04 ± 1.76	22.01 ± 1.45	17.38 ± 1.15
Flower bud 12 mm	15.30 ± 0.64	19.33 ± 0.83	24.03 ± 0.52	21.69 ± 0.13	20.03 ± 0.65	21.54 ± 0.62	21.41 ± 0.96	19.51 ± 0.77	15.98 ± 0.45
C	GhACT4	GhEF1α 5	GhFBX6	GhPP2A1	GhMZA	GhPTB	GhGAPC2	GhβTUB3	GhUBQ14
Efficiency ± SD	0.97 ± 0.021	0.92 ± 0.029	0.94 ± 0.017	0.82 ± 0.019	0.92 ± 0.024	0.91 ± 0.031	0.88 ± 0.032	0.93 ± 0.009	0.96 ± 0.024
Floral organs	Cq ± SD
Carpels	17.34 ± 0.52	17.16 ± 1.18	24.11 ± 0.73	22.31 ± 0.66	20.85 ± 0.40	21.93 ± 0.77	22.14 ± 1.60	21.20 ± 0.28	16.12 ± 0.63
Stames	16.87 ± 0.29	16.08 ± 0.19	24.37 ± 0.09	22.12 ± 0.59	21.59 ± 0.31	21.78 ± 0.70	22.76 ± 0.53	21.33 ± 0.20	17.77 ± 0.29
Sepals	16.33 ± 0.39	15.82 ± 0.63	23.08 ± 0.36	21.96 ± 0.47	20.66 ± 0.19	21.50 ± 0.18	23.24 ± 0.12	20.31 ± 0.20	16.17 ± 0.85
Petals	18.08 ± 2.00	18.55 ± 2.52	25.39 ± 1.37	23.17 ± 0.79	22.65 ± 1.72	23.51 ± 1.56	24.09 ± 0.13	21.25 ± 1.93	18.51 ± 1.99
Pedicels	16.56 ± 0.19	16.11 ± 0.32	25.02 ± 0.85	23.69 ± 0.11	22.52 ± 0.92	23.28 ± 0.72	22.25 ± 0.56	21.60 ± 0.08	16.28 ± 0.33
D	GhACT4	GhEF1α 5	GhFBX6	GhPP2A1	GhMZA	GhPTB	GhGAPC2	GhβTUB3	GhUBQ14
Efficiency ± SD	0.96 ± 0.019	0.94 ± 0.17	1.01 ± 0.012	0.94 ± 0.017	1.01 ± 0.018	0.98 ± 0.014	0.96 ± 0.018	0.94 ± 0.026	0.93 ± 0.020
Fruits	Cq ± SD
Fruits 10-15 mm	16.78 ± 0.74	18.56 ± 1.36	26.33 ± 0.30	23.43 ± 1.00	22.44 ± 0.65	24.13 ± 0.57	27.85 ± 0.51	20.67 ± 0.27	17.52 ± 0.15
Fruits 16-20 mm	17.27 ± 0.19	18.49 ± 1.17	26.64 ± 0.93	22.78 ± 1.10	20.89 ± 0.07	23.12 ± 0.48	26.79 ± 0.70	19.61 ± 0.42	17.28 ± 0.26
Fruits 21-30 mm	17.39 ± 0.47	18.89 ± 0.14	26.09 ± 0.75	23.34 ± 0.21	21.45 ± 0.28	22.75 ± 0.98	27.39 ± 0.67	20.14 ± 1.30	17.17 ± 0.18
Fruits >30 mm	19.89 ± 1.58	20.89 ± 1.78	29.17 ± 2.12	24.61 ± 0.72	23.06 ± 0.72	24.70 ± 0.46	26.94 ± 2.49	20.64 ± 1.37	18.70 ± 1.15

The values of efficiency of primers were generated for each experimental situation (A-plant organs, B-flower buds, C-floral organs and D-fruit).

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ISSN: 1471-2229

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